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Data sheet of PRKCA (phospho S657/Y658) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Rabbit,Rat |
| Host species | Rabbit |
| Applications | WB,IP,ELISA |
More info about PRKCA (phospho S657/Y658) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB7910 |
| Product name: | PRKCA (phospho S657/Y658) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKCA. |
| Gene id: | 5578 |
| Gene name: | PRKCA |
| Gene alias: | AAG6|MGC129900|MGC129901|PKC-alpha|PKCA|PRKACA |
| Gene description: | protein kinase C, alpha |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S657/Y658 of human PRKCA. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000) Immunoprecipitation (5-10 ul) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition, |
| Reactivity: | Human,Rabbit,Rat |
| Application image: |  |
| Application image note: | Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PRKCA antibody. Control (lane 1) and alkaline phosphatase treated (lane 2) precipitates were probed with PRKCA (phospho S657/Y658) polyclonal antibody (Cat # PAB7910). The latter shows no detection of PRKCA after phosphatase treatment. |
| Applications: | WB,IP,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | The molecular heterogeneity of protein kinase C and its implications for cellular regulation.Nishizuka Y. Nature. 1988 Aug 25;334(6184):661-5. |