|Product name:||Exosome Standard (HCT116 cell line)|
|Product description:||This product is purified and lyophilized exosomes obtained from HCT116 cell line (human colon carcinoma) and can be used as control standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples.|
|Preparation method:||Exosomes are purified following a combination of ultracentrifugation and microfiltration steps. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis). Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids).|
|Storage buffer:||Lyophilized from cell culture supernatant.|
|Storage instruction:||Lyophilized exosome standards can be stored at 4Â°C for up to 36 months.|
Reconstituted exosome standards are not suitable for long-term storage at room temperature, use them within 2 hours after reconstitution.
The remaining reconstituted standard stock solution should be aliquoted into low binding polypropylene vials and stored at -20Â°C for up to 1 month or at -80Â°C for up to 6 months.
Strictly avoid repeated freeze-and-thaw cycles.
|Quality control testing:||Comparison of exosomal markers on fresh and lyophilized exosomes.|
|Quality control testing picture:|
|Note:||Reconstitute lyophilized exosome standard by adding deionized water. 30 uL for lyophilized standard 30 ug to get a final concentration of 1 ug/uL. Different volumes of deionized water for exosomes reconstitution can be chosen by the users in according with the desired final concentration. Suspend exosomes pipetting the solution up and down 10-15 times and avoid bubbles. Vortex the reconstituted standard for 60 seconds. Briefly centrifuge the tubes containing the standard to ensure that the solution is collected at the bottom of the tube. Pipette the solution up and down 10 times, avoiding the introduction of bubbles. After this step, the standard is ready to use.|
|Shipping condition:||Dry Ice|