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Data sheet of Histone H4 (K20me3) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | ELISA,ChIP,ChIP-Seq,Dot,WB-Ce,IF |
More info about Histone H4 (K20me3) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31329 |
| Product name: | Histone H4 (K20me3) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H4 (K20me3). |
| Gene id: | 8359 |
| Gene name: | HIST1H4A |
| Gene alias: | H4/a|H4FA |
| Gene description: | histone cluster 1, H4a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H4, trimethylated at lysine 20. |
| Protein accession: | P62805 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100) Western Blot (1:1000) ChIP (1-2 ug/IP) Dot Blot (1:20000) Immunofluorescence (1:300) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoters of the active genes c-fos and GAPDH, used as negative controls, and for the Sat2 satellite repeat region used as a positive control. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). |
| Applications: | ELISA,ChIP,ChIP-Seq,Dot,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Heat shock represses rRNA synthesis by inactivation of TIF-IA and lncRNA-dependent changes in nucleosome positioning.Zhao Z, Dammert MA, Hoppe S, Bierhoff H, Grummt I. Nucleic Acids Res. 2016 Sep 30;44(17):8144-52. |