| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | ELISA,ChIP,Dot,WB-Ce,IF |
| Brand: | Abnova |
| Reference: | PAB31276 |
| Product name: | Histone H4 (K8ac) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H4 (K8ac). |
| Gene id: | 8359 |
| Gene name: | HIST1H4A |
| Gene alias: | H4/a|H4FA |
| Gene description: | histone cluster 1, H4a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H4, acethylated at lysine 20. |
| Protein accession: | P62805 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100) Western Blot (1:200) ChIP (1 ug/CHIP) Dot Blot (1:20000) Immunofluorescence (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: | ![]() |
| Application image note: | ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Histone H4 (K8ac), crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:16700. |
| Applications: | ELISA,ChIP,Dot,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Citrate modulates lipopolysaccharide-induced monocyte inflammatory responses.Ashbrook MJ, McDonough KL, Pituch JJ, Christopherson PL, Cornell TT, Selewski DT, Shanley TP, Blatt NB. Clin Exp Immunol.2015 Jun;180(3):520-30. |