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Data sheet of Histone H3 (K9/14ac) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rainbow trout,Zebra fish,A. Nidulans,Arabidopsis |
| Host species | Rabbit |
| Applications | ELISA,ChIP,ChIP-Seq,Dot,WB-Ce,IF |
More info about Histone H3 (K9/14ac) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31269 |
| Product name: | Histone H3 (K9/14ac) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K9/14ac). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, acetylated lysines at 9 and 14. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100) Western Blot (1:1000) ChIP (1-2 ug/CHIP) Dot Blot (1:20000) Immunofluorescence (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse,Rainbow trout,Zebra fish,A. Nidulans,Arabidopsis |
| Application image: |  |
| Application image note: | Cross reactivity tests using the Histone H3 (K9/14ac) antibody. Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20000. The figure shows a high specificity of the antibody for the modification of interest. |
| Applications: | ELISA,ChIP,ChIP-Seq,Dot,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | H3.3 demarcates GC-rich coding and subtelomeric regions and serves as potential memory mark for virulence gene expression in Plasmodium falciparum.Fraschka SA et al. Sci Rep.2016 Aug 24;6:31965. |