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Data sheet of Histone H3 (K4me3) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Rainbow trout |
| Host species | Rabbit |
| Applications | ELISA,ChIP,Dot,WB-Ce |
More info about Histone H3 (K4me3) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB31263 |
| Product name: | Histone H3 (K4me3) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me3). |
| Gene id: | 8350 |
| Gene name: | HIST1H3A |
| Gene alias: | H3/A|H3FA |
| Gene description: | histone cluster 1, H3a |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Histone H3, trimethylated at lysine 4. |
| Protein accession: | P68431 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100-500) Western Blot (1:1000) ChIP (1 ul/CHIP) Dot Blot (1:20000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide). |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Tag: | KLH |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Rainbow trout |
| Application image: |  |
| Application image note: | ChIP assays were performed using human U2OS cells. A titration consisting of 1, 5 and 10 ul of antibody per ChIP experiment was analyzed. IgG (1 ug/ IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the constitutively expressed GAPDH gene and for myoglobin exon 2. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes. |
| Applications: | ELISA,ChIP,Dot,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | c-Myc Antagonises the Transcriptional Activity of the Androgen Receptor in Prostate Cancer Affecting Key Gene Networks.Stefan J. Barfeld, Alfonso Urbanucci, Harri M. Itkonen, Ladan Fazli , Jessica L. Hicks , Bernd Thiede , Paul S. Rennie , Srinivasan Yegnasubramanian, Angelo M. DeMarzo , Ian G. Mills. EBioMedicine.2017 Apr;18:83-93. |