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Data sheet of Histone H2B (ubiquitinated) monoclonal antibody, clone NRO3
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Fruit fly,Human,Mouse,Arabidopsis |
| Host species | Mouse |
| Applications | ChIP,IHC-P,IF,WB-Tr |
More info about Histone H2B (ubiquitinated) monoclonal antibody, clone NRO3
| Brand: | Abnova |
| Reference: | MAB10006 |
| Product name: | Histone H2B (ubiquitinated) monoclonal antibody, clone NRO3 |
| Product description: | Mouse monoclonal antibody raised against synthetic peptide of Histone H2B (ubiquitinated). |
| Clone: | NOR3 |
| Isotype: | IgG2a |
| Immunogen: | A branched peptide corresponding to the conjugation site of ubiquitin on human Histone H2B. |
| Form: | Lyophilized |
| Recommend dilutions: | Immunohistochemistry (1:200-500) Western Blot (2-3 ug/mL) ChIP (3-5 ug/CHIP) Immunofluorescence The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 2 ug/ul solution in PBS, pH 7.4. |
| Storage instruction: | Store at 4°C for three months. For long term storage store at -20°C. After reconstitution with distilled water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | The antibody recognizes the K120 ubiquitination of the human histone H2B with no detectable cross-reactivity with either non-ubiquitinated H2B or ubiquitinated. |
| Reactivity: | Fruit fly,Human,Mouse,Arabidopsis |
| Application image: |  |
| Application image note: | Western Blot (Cell lysate) analysis of (1) HeLa cells transfected with siRNA directed against LacZ, (2) siRNA directed against the H2B E3 ubiquitin ligase (hBRE1). |
| Applications: | ChIP,IHC-P,IF,WB-Tr |
| Shipping condition: | Dry Ice |