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Data sheet of Acetylated-TP53 Lys120 monoclonal antibody, clone 10E5
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | WB,IP,ELISA |
More info about Acetylated-TP53 Lys120 monoclonal antibody, clone 10E5
| Brand: | Abnova |
| Reference: | MAB0661 |
| Product name: | Acetylated-TP53 Lys120 monoclonal antibody, clone 10E5 |
| Product description: | Mouse monoclonal antibody raised against synthetic peptides of TP53. |
| Clone: | 10E5 |
| Isotype: | IgG1, kappa |
| Gene id: | 7157 |
| Gene name: | TP53 |
| Gene alias: | FLJ92943|LFS1|TRP53|p53 |
| Gene description: | tumor protein p53 |
| Immunogen: | Synthetic acylated peptide (conjugated with carrier protein) corresponding to residues surrounding K120 of human TP53. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.4 (50% glycerol) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Identification of Acetylated-TP53 Lys120 monoclonal antibody, clone 10E5 (Cat # MAB0661) by Western blotting with 10E5 monoclonal antibody. Samples are crude lysates of HCT116 cells. Left lanes are control. Right lanes are cells treated with siRNA to knockdown the expression of a Tip60 interacting protein. Total p53 was immunoprecipitated with omnipotent anti-p53 monoclonal antibody from the crude extracts and analyzed by western blotting with anti-p53 antibody (upper panel) or anti-p53 acetyl-K120 monoclonal antibody (10E5) (middle panel) . The lower panel shows total p53. |
| Applications: | WB,IP,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | Induction of tumor-reactive T helper responses by a posttranslational modified epitope from tumor protein p53.Kumai T, Ishibashi K, Oikawa K, Matsuda Y, Aoki N, Kimura S, Hayashi S, Kitada M, Harabuchi Y, Celis E, Kobayashi H Cancer Immunol Immunother. 2014 May;63(5):469-78. doi: 10.1007/s00262-014-1533-z. Epub 2014 Mar 15. |