Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of Mouse Monoclonal to Lyn
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Mouse, Human, Rat |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to Lyn
| Brand: | Exbio |
| Product no.: | 11-377-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to Lyn |
| Antigen: | Lyn |
| Clonality: | Monoclonal |
| Clone: | LYN-01 |
| Isotype: | IgG1 |
| Immunogen: | Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238). |
| Format: | purified |
| Specificity: | The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues. |
| Categories: | Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Rodent), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Kovarova M, Tolar P, Arudchandran R, Draberova L, Rivera J, Draber P.: Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation. Mol Cell Biol. 2001 Dec;21(24):8318-28. _x000D_ , *Amoui M, Draberova L, Tolar P, Draber P.: Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur J Immunol. 1997 Jan;27(1):321-8. _x000D_ , *Draberova L, Amoui M, Draber P.: Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane_x000D_ microdomains. Immunology. 1996 Jan;87(1):141-8. _x000D_ |
| General references: | *Dos Santos C, Demur C, Bardet V, Prade-Houdellier N, Payrastre B, Récher C: A critical role for Lyn in acute myeloid leukemia. Blood. 2008 Feb 15;111(4):2269-79._x000D_ _x000D_ , *Kinoshita T, Miyano N, Nakai R, Yokota K, Ishiguro H, Tada T: Protein purification and preliminary crystallographic analysis of human Lyn tyrosine kinase. Protein Expr Purif. 2008 Apr;58(2):318-24. _x000D_ _x000D_ , *Davey AM, Krise KM, Sheets ED, Heikal AA: Molecular perspective of antigen-mediated mast cell signaling. J Biol Chem. 2008 Mar 14;283(11):7117-27._x000D_ _x000D_ , *Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20._x000D_ _x000D_ _x000D_ , *Strzelecka-Kiliszek A, Kwiatkowska K, Sobota A: Lyn and Syk kinases are sequentially engaged in phagocytosis mediated by Fc gamma R. J Immunol. 2002 Dec 15;169(12):6787-94._x000D_ _x000D_ _x000D_ |
| Related products: | - Mouse Monoclonal to MAP2ab - Mouse Monoclonal to Lysozyme - Mouse Monoclonal to MAP2 |
| Shipping condition: | Room temperature |