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Data sheet of Mouse Monoclonal to LAT
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Mouse, Rat |
| Clonality | Monoclonal |
| Host species | Mouse |
| Applications | WB, IP, ICC |
More info about Mouse Monoclonal to LAT
| Brand: | Exbio |
| Product no.: | 11-442-C025 |
| Product name: | Mouse Monoclonal to LAT |
| Antigen: | LAT |
| Clonality: | Monoclonal |
| Negative species: | Human |
| Clone: | LAT-1D1 |
| Isotype: | IgG2a |
| Immunogen: | Bacterially expressed full-length rat LAT |
| Format: | purified |
| Specificity: | The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. |
| Categories: | Adaptor Proteins (Rodent), Adaptor Proteins |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Product specific references: | *Volná P, Lebduska P, Dráberová L, SÃmová S, Heneberg P, BoubelÃk M, Bugajev V, Malissen B, Wilson BS, Horejsà V, Malissen M, Dráber P: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13., *Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51. , *Heneberg P, Lebduska P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806. , *Tolar P, Tumova M, Draber P: New monoclonal antibodies recognizing the adaptor protein LAT. Folia Biol (Praha). 2001;47(6):215-7. |
| General references: | Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15. |
| Shipping condition: | Room temperature |