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Data sheet of Mouse Monoclonal to H-Ras
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human, Other not determined |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to H-Ras
| Brand: | Exbio |
| Product no.: | 11-527-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to H-Ras |
| Antigen: | H-Ras |
| Clonality: | Monoclonal |
| Clone: | H-RAS-03 |
| Isotype: | IgG1 |
| Immunogen: | Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein_x000D_ |
| Format: | purified |
| Specificity: | The antibody H-RAS-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals. _x000D_ |
| Categories: | Signaling of Small GTPases (Human), Signaling of Small GTPases |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation._x000D_ |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| General references: | *Rotblat B, Prior IA, Muncke C, Parton RG, Kloog Y, Henis YI, Hancock JF: Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane. Mol Cell Biol. 2004 Aug;24(15):6799-810., *Prior IA, Harding A, Yan J, Sluimer J, Parton RG, Hancock JF: GTP-dependent segregation of H-ras from lipid rafts is required for biological activity. Nat Cell Biol. 2001 Apr;3(4):368-75._x000D_ , *Rotblat B, Yizhar O, Haklai R, Ashery U, Kloog Y: Ras and its signals diffuse through the cell on randomly moving nanoparticles. Cancer Res. 2006 Feb 15;66(4):1974-81._x000D_ , *Roy S, Plowman S, Rotblat B, Prior IA, Muncke C, Grainger S, Parton RG, Henis YI, Kloog Y, Hancock JF: Individual palmitoyl residues serve distinct roles in H-ras trafficking,_x000D_ microlocalization, and signaling. Mol Cell Biol. 2005 Aug;25(15):6722-33._x000D_ , *Zheng Y, Quilliam LA: Activation of the Ras superfamily of small GTPases. Workshop on exchange factors. EMBO Rep. 2003 May;4(5):463-8._x000D_ |
| Related products: | - Mouse Monoclonal to HSV1 (glycoprotein C) - Mouse Monoclonal to Hsp90 alpha,beta - Mouse Monoclonal to Hsp90 beta |
| Shipping condition: | Room temperature |