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Data sheet of Mouse Monoclonal to HLA-Class I
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to HLA-Class I
| Brand: | Exbio |
| Product no.: | 11-242-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to HLA-Class I |
| Antigen: | HLA-Class I |
| Clonality: | Monoclonal |
| Clone: | MEM-147 |
| Isotype: | IgG1 |
| Immunogen: | PHA-activated peripheral blood lymphocytes |
| Format: | purified |
| Specificity: | The antibody MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. _x000D_ The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32. |
| Categories: | MHC (Major Histocompatibility Complex) and Related Antigens (Human) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck_x000D_ and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8., *Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32_x000D_ and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6. _x000D_ |
| Related products: | - Mouse Monoclonal to HLA-DR+DP - Mouse Monoclonal to HLA-DQ1+DQ3 - Mouse Monoclonal to HLA-DR |
| Shipping condition: | Room temperature |