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Data sheet of Mouse Monoclonal to Acrosin (Porcine)
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Porcine |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to Acrosin (Porcine)
| Brand: | Exbio |
| Product no.: | 11-260-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to Acrosin (Porcine) |
| Antigen: | Acrosin (Porcine) |
| Clonality: | Monoclonal |
| Negative species: | Human, Canine (Dog), Bovine |
| Clone: | ACR-2 |
| Isotype: | IgG1 |
| Immunogen: | Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization. |
| Format: | purified |
| Specificity: | The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa. _x000D_ _x000D_ |
| Categories: | Fertility & Reproduction (Veterinary), Fertility & Reproduction |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J: Effect of estrogens on boar sperm capacitation in vitro. Reprod Biol Endocrinol. 2010 Jul 13;8:87, *Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15;56(2):211-23. _x000D_ , *Moos J, Peknicova J, Tesarik J. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug;49(2):408-15. _x000D_ , *Moos J, Peknicova J, Tesarik J. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16;1176(3):199-207., *Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct;22(5):427-35. _x000D_ , *Moos J, Tesarik J, Leca G, Peknicova J. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2;294(1-2):27-30. |
| General references: | *Peknicová J. et al., Anim. Reprod. Sci. 35, 255 (1994). |
| Related products: | - Mouse Monoclonal to AGR2+AGR3 - Mouse Monoclonal to Actin (muscle) - Mouse Monoclonal to AGPS |
| Shipping condition: | Room temperature |