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| Brand | Abnova |
| Product type | Primary antibodies |
| Clonality | Monoclonal |
| Host species | Rabbit |
| Applications | ELISA,ICC,Dot,IHC-P,MeDIP |
| Product description: | Rabbit monoclonal antibody raised against 5-hydroxymethylcytosine (5-hmC). |
| Clone: | RM236 |
| Isotype: | IgG |
| Immunogen: | 5-hydroxymethylcytosine conjugated with BSA. |
| Form: | Liquid |
| Recommend dilutions: | Dot Blot (0.2-1 ug/mL) ELISA (0.1-1 ug/mL) Immunocytochemistry (0.5-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-1 ug/mL) Hydroxymethylated DNA immunoprecipitation (0.2-2 ug/mL) The optimal working dilu |
| Storage buffer: | In PBS (50% glycerol, 1% BSA, 0.09% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Specificity: | This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA. |
| Size: | 50 ug |
| Shipping condition: | Dry Ice |