| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Pig,Rat |
| Clonality | Monoclonal |
| Host species | Mouse |
| Applications | IHC-P,Flow Cyt |
| Product description: | Mouse monoclonal antibody raised against native pig NEFH. |
| Clone: | NE14 |
| Isotype: | IgG1 |
| Immunogen: | Crude neurofilament preparation from porcine spinal cord. |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM PBS. |
| Storage instruction: | Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing. |
| Specificity: | This monoclonal antibody identified as heavy subunit of neurofilaments (NEFH). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this antibody no longer binds. |
| Size: | 100 ug |
| Shipping condition: | Dry Ice |