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Data sheet of Phosphotyrosine monoclonal antibody, clone E10
| Brand | Abnova |
| Product type | Primary antibodies |
| Clonality | Monoclonal |
| Host species | Mouse |
| Applications | IP,ELISA,ICC,WB-Ce,IF,Flow Cyt |
More info about Phosphotyrosine monoclonal antibody, clone E10
| Product description: | Mouse monoclonal antibody raised against Phosphotyrosine. |
| Clone: | E10 |
| Isotype: | IgG1, kappa; IgG2a, kappa |
| Immunogen: | Phosphotyrosine containing carrier protein. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (0.1 ug/mL) Flow Cytometry (4 ug/mL) Immunocytochemistry (4 ug/mL) Immunofluorescence (4 ug/mL) Immunoprecipitation (1-4 ug/mL) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide). |
| Storage instruction: | Store at -20°C or lower. Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL. The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Specificity: | The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues. |
| Size: | 100 ug |
| Shipping condition: | Dry Ice |