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Data sheet of CD45RA monoclonal antibody, clone PTPRC/818
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Monoclonal |
| Host species | Mouse |
| Applications | IHC-P,IF,Flow Cyt |
More info about CD45RA monoclonal antibody, clone PTPRC/818
| Product description: | Mouse monoclonal antibody raised against full length recombinant human CD45RA. |
| Clone: | PTPRC/818 |
| Isotype: | IgG2a, kappa |
| Gene id: | 5788 |
| Gene name: | PTPRC |
| Gene alias: | B220|CD45|CD45R|GP180|LCA|LY5|T200 |
| Gene description: | protein tyrosine phosphatase, receptor type, C |
| Immunogen: | Recombinant protein corresponding to full length of human CD45RA. |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (0.5-1 ug/million cells in 0.1 mL) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM PBS (0.05% BSA, 0.05% sodium azide). |
| Storage instruction: | Store at 4°C. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Specificity: | Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve). |
| Size: | 100 ug |
| Shipping condition: | Blue Ice |