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Data sheet of VHL monoclonal antibody, clone AT82B10
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | ELISA,WB-Ce,IF |
More info about VHL monoclonal antibody, clone AT82B10
| Brand: | Abnova |
| Reference: | MAB1074 |
| Product name: | VHL monoclonal antibody, clone AT82B10 |
| Product description: | Mouse monoclonal antibody raised against partial recombinant VHL. |
| Clone: | AT82B10 |
| Isotype: | IgG2b, kappa |
| Gene id: | 7428 |
| Gene name: | VHL |
| Gene alias: | HRCA1|RCA1|VHL1 |
| Gene description: | von Hippel-Lindau tumor suppressor |
| Genbank accession: | NM_000551 |
| Immunogen: | Recombinant protein corresponding to amino acids 1-154 of human VHL from E. coli. |
| Protein accession: | NP_000542 |
| Form: | Liquid |
| Recommend dilutions: | ELISA Immunofluorescence (1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.4 (10% glycerol, 0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunofluorescent staining of Hep3B cell line with antibody. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis.Maxwell PH, Wiesener MS, Chang GW, Clifford SC, Vaux EC, Cockman ME, Wykoff CC, Pugh CW, Maher ER, Ratcliffe PJ. Nature. 1999 May 20;399(6733):271-5. |