| Brand: | Abnova |
| Reference: | MAB0960 |
| Product name: | LIME1 monoclonal antibody, clone LIME-06 |
| Product description: | Mouse monoclonal antibody raised against partial recombinant LIME1. |
| Clone: | LIME-06 |
| Isotype: | IgG1 |
| Gene id: | 54923 |
| Gene name: | LIME1 |
| Gene alias: | FLJ20406|LIME|LP8067|dJ583P15.4 |
| Gene description: | Lck interacting transmembrane adaptor 1 |
| Immunogen: | Recombinant protein corresponding to amino acids 141-295 of human LIME1. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.4 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 KDa Lck-interacting transmembrane adaptor expressed by T cells. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Induction of LIME1 tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME1 was immunoprecipitated from laurylmaltoside lysates with the LIME1 monoclonal antibody, clone LIME-06 (Cat # MAB0960) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME1 (top) and total LIME1 (bottom). |
| Applications: | IP |
| Shipping condition: | Blue Ice |