| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | ELISA,WB-Ce,WB-Re |
| Brand: | Abnova |
| Reference: | MAB0011 |
| Product name: | TP53 (phospho S46) monoclonal antibody, clone TK-4D4 |
| Product description: | Mouse monoclonal antibody raised against synthetic phosphopeptide of TP53. |
| Clone: | TK-4D4 |
| Isotype: | IgG1 |
| Gene id: | 7157 |
| Gene name: | TP53 |
| Gene alias: | FLJ92943|LFS1|TRP53|p53 |
| Gene description: | tumor protein p53 |
| Genbank accession: | X60014 |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S46 of human TP53. |
| Protein accession: | CAA42629 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1-2 ug/mL) ELISA (1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50% glycerol) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Phospho-p53 Ser46 Antibody detects phosphorylated p53 at serine 46 in an immunoprecipitates using anti-p53 specific polyclonal antibody, by western blotting or phosphorylated recombinant p53 only when phosphorylated at serine 46 in vitro, by western blotting. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | H2O2 and Adriamycin treatment induce phosphorylation of p53 at serine 46 in MCF-7 cells which expresses wild type p53. Detected by Immunoprecipitation-western blotting using anti-p53 polyclonal antibody and p53 (phospho S46) monoclonal antibody, clone TK-4D4 (Cat # MAB0011). |
| Applications: | ELISA,WB-Ce,WB-Re |
| Shipping condition: | Dry Ice |