EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured) View larger

EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

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Data sheet of EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

BrandAbnova
Product typeLysate
Host speciesHuman
ApplicationsWB,IP

More info about EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

Brand: Abnova
Reference: L080T6
Product name: EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
Gene id: 1906
Gene name: EDN1
Gene alias: ET1|HDLCQ7
Gene description: endothelin 1
Genbank accession: BC009720
Protein accession: AAH09720
Concentration: 2 mg/ml
Plasmid: pCMV-EDN1 full length
Lysis buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Host cell: HEK293
Recommend dilutions: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage buffer: In modified RIPA Lysis Buffer.
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: Transient overexpression cell lysate was tested with Anti-EDN1 antibody (H00001906-M01) by Western Blots.
Quality control testing picture: qc_test-L080T6-1.jpg
Quality control testing picture note: EDN1 transfected lysate
Product type: Lysate
Host species: Human
Antigen species / target species: Human
Applications: WB,IP
Shipping condition: Dry Ice

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