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Data sheet of AFP HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
| Brand | Abnova |
| Product type | Lysate |
| Host species | Human |
| Applications | WB,IP |
More info about AFP HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
| Brand: | Abnova |
| Reference: | L006T6 |
| Product name: | AFP HEK293 Cell Transient Overexpression Lysate(Non-Denatured) |
| Gene id: | 174 |
| Gene name: | AFP |
| Gene alias: | FETA|HPAFP |
| Gene description: | alpha-fetoprotein |
| Genbank accession: | BC027881 |
| Protein accession: | AAH27881 |
| Concentration: | 2 mg/ml |
| Plasmid: | pCMV-AFP full length |
| Lysis buffer: | Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Host cell: | HEK293 |
| Recommend dilutions: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage buffer: | In modified RIPA Lysis Buffer. |
| Storage instruction: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Transient overexpression cell lysate was tested with Anti-AFP antibody (H00000174-M01) by Western Blots. |
| Quality control testing picture: |  |
| Quality control testing picture note: | AFP transfected lysate |
| Product type: | Lysate |
| Host species: | Human |
| Antigen species / target species: | Human |
| Applications: | WB,IP |
| Shipping condition: | Dry Ice |