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Data sheet of Intracellular Total ROS Activity Assay Kit (Green Fluorescence)
| Brand | Abnova |
| Product type | Elisa & Assay kits |
| Applications | Quant |
More info about Intracellular Total ROS Activity Assay Kit (Green Fluorescence)
| Brand: | Abnova |
| Reference: | KA4074 |
| Product name: | Intracellular Total ROS Activity Assay Kit (Green Fluorescence) |
| Product description: | Intracellular Total ROS Activity Assay Kit (Green Fluorescence) is used for the measurement of intracellular total ROS activity. |
| Storage instruction: | Store the kit in desiccated environment at -20°C and avoid from light. |
| Quality control testing picture: |  |
| Quality control testing picture note: | Jurkat cells were seeded on the same day at 300,000 cells/100uL/well in a Costar black wall/clear bottom 96-well plate. The ROS assay loading solution (100 uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. And then the cells were treated with 1mM, 0.1mM H2O2 or without H2O2 for 30 minutes. The fluorescence signal was monitored at Ex/Em = 490/525 nm (cutoff at 515 nm) with bottom read mode using FlexStation (Molecular Devices). |
| Note: | Detection of ROS in Jurkat cells. |
| Product type: | Elisa & Assay kits |
| Applications: | Quant |
| Shipping condition: | Dry Ice |