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Data sheet of WT1 recombinant monoclonal antibody, clone rWT1/857
| Brand | Abnova |
| Reactivity | Human |
| Applications | IHC-P,IF,Flow Cyt |
More info about WT1 recombinant monoclonal antibody, clone rWT1/857
| Brand: | Abnova |
| Reference: | RAB00273 |
| Product name: | WT1 recombinant monoclonal antibody, clone rWT1/857 |
| Product description: | Recombinant mouse monoclonal antibody raised against human WT1. |
| Clone: | rWT1/857 |
| Isotype: | IgG2b, kappa |
| Gene id: | 7490 |
| Gene name: | WT1 |
| Gene alias: | GUD|WAGR|WIT-2|WT33 |
| Gene description: | Wilms tumor 1 |
| Immunogen: | Original antibody is raised against recombinant protein corresponding to full length human WT1. |
| Protein accession: | P19544 |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (0.5-1 ug/million cells) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM PBS (0.05% BSA and 0.05% azide). |
| Storage instruction: | Store at 4°C. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Antigen species / target species: | Human |
| Specificity: | Recognizes a 47-55kDa tumor suppressor protein which identified as WT1 protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with WT1 recombinant monoclonal antibody, clone rWT1/857 (Cat # RAB00273). |
| Applications: | IHC-P,IF,Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | The WT1 Wilms tumor gene product: a developmentally regulated transcription factor in the kidney that functions as a tumor suppressor.Rauscher FJ 3rd. FASEB J. 1993 Jul;7(10):896-903. |