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Data sheet of TDGF1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | WB,ELISA,IHC |
More info about TDGF1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9995 |
| Product name: | TDGF1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of TDGF1. |
| Gene id: | 6997 |
| Gene name: | TDGF1 |
| Gene alias: | CR|CRGF|CRIPTO|Cripto-1 |
| Gene description: | teratocarcinoma-derived growth factor 1 |
| Immunogen: | A synthetic peptide corresponding to internal region of human TDGF1. |
| Protein accession: | NP_003203;P13385 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:105000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | Western blot using TDGF1 polyclonal antibody (Cat # PAB9995) shows detection of endogenous and transfected TDGF1 from mouse and human sources. The TDGF1 band appears above the 17.8 KDa marker. Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse TDGF1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose TDGF1 expression. COS-7 cells transfected with human TDGF1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control. A non-specific band at 45 KDa may be present in some preparations. The primary antibody was used at a 1 : 500 dilution. Personal communication, C. Bianco, NCI, Bethesda, MD. |
| Applications: | WB,ELISA,IHC |
| Shipping condition: | Dry Ice |
| Publications: | Cripto-1: an oncofetal gene with many faces.Bianco C, Strizzi L, Normanno N, Khan N, Salomon DS. Curr Top Dev Biol. 2005;67:85-133. |