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Data sheet of CDC27 (phospho T244) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about CDC27 (phospho T244) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9988 |
| Product name: | CDC27 (phospho T244) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC27. |
| Gene id: | 996 |
| Gene name: | CDC27 |
| Gene alias: | APC3|CDC27Hs|D0S1430E|D17S978E|HNUC |
| Gene description: | cell division cycle 27 homolog (S. cerevisiae) |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T244 of human CDC27. |
| Protein accession: | P30260;NP_001247 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:8000-1:30000) Western Blot (1:300-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human CDC27 pT244 protein and This antibody is specific to the phosphorylated form of the protein. This antibody does not cross-react with CDC27 phosphorylated at other sites. |
| Reactivity: | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using CDC27 (phospho T244) polyclonal antibody (Cat # PAB9988) shows detection of a band ~92 KDa corresponding to phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis. Each lane contains approximately 30 ug of HeLa whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. ECL reagent was used for detection. Data contributed by Bing Li, UT South western. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Mad2 transiently associates with an APC/p55Cdc complex during mitosis.Wassmann K, Benezra R. Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11193-8. |