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Data sheet of CDC27 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IF |
More info about CDC27 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9986 |
| Product name: | CDC27 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of CDC27. |
| Gene id: | 996 |
| Gene name: | CDC27 |
| Gene alias: | APC3|CDC27Hs|D0S1430E|D17S978E|HNUC |
| Gene description: | cell division cycle 27 homolog (S. cerevisiae) |
| Immunogen: | A synthetic peptide corresponding to amino acids 422-430 of human CDC27. |
| Protein accession: | P30260;NP_001247 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:40000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using CDC27 polyclonal antibody (Cat # PAB9986) shows detection of a band ~90 KDa corresponding to human CDC27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 2 h atroom temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developedby LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Mad2 transiently associates with an APC/p55Cdc complex during mitosis.Wassmann K, Benezra R. Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11193-8. |