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Data sheet of CAMK2G polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Dog,Frog,Human,Mouse,Orangutan,Pig,Rabbit,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ti |
More info about CAMK2G polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9984 |
| Product name: | CAMK2G polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of CAMK2G. |
| Gene id: | 818 |
| Gene name: | CAMK2G |
| Gene alias: | CAMK|CAMK-II|CAMKG|FLJ16043|MGC26678 |
| Gene description: | calcium/calmodulin-dependent protein kinase II gamma |
| Immunogen: | A synthetic peptide corresponding to amino acids 6-23 of human CAMK2G. |
| Protein accession: | Q9UQM7(Human);P11798(Mouse);P11275(Rat) |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:20000) Western Blot (1:1000-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chicken,Dog,Frog,Human,Mouse,Orangutan,Pig,Rabbit,Rat |
| Application image: |  |
| Application image note: | Western blot using CAMK2G polyclonal antibody (Cat # PAB9984) shows detectionof a band ~54 KDa corresponding to human CAMK2G (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2). Approximately 35 ug of a mouse brain tissue lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CAMK2G was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademarkof LI-COR, Inc. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Ca2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity.Thiel G, Czernik AJ, Gorelick F, Nairn AC, Greengard P. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6337-41. |