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Data sheet of BMI1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Cat,Chicken,Chimpanzee,Clawed frog,Dog,Human,Orangutan,Rat |
| Host species | Goat |
| Applications | ELISA,WB-Ce |
More info about BMI1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9982 |
| Product name: | BMI1 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of BMI1. |
| Gene id: | 648 |
| Gene name: | BMI1 |
| Gene alias: | MGC12685|PCGF4|RNF51 |
| Gene description: | BMI1 polycomb ring finger oncogene |
| Immunogen: | A synthetic peptide corresponding to amino acids 252-264 of human BMI1. |
| Protein accession: | NP_005171;P35226 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:30000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Cat,Chicken,Chimpanzee,Clawed frog,Dog,Human,Orangutan,Rat |
| Application image: |  |
| Application image note: | Western blot using BMI1 polyclonal antibody (Cat # PAB9982) shows detection of a band ~37 KDa corresponding to human BMI1 (arrowhead). Approximately 20 ug of a U-2 OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase.Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K. Genes Cells. 2004 Feb;9(2):105-20. |