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Data sheet of ACTB polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB,ELISA,IF |
More info about ACTB polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9980 |
| Product name: | ACTB polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ACTB. |
| Gene id: | 60 |
| Gene name: | ACTB |
| Gene alias: | PS1TP5BP1 |
| Gene description: | actin, beta |
| Immunogen: | A synthetic peptide corresponding to amino acids 359-368 of human ACTB. |
| Protein accession: | P60709;NP_001092 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:1000-1:4000) Immunofluorescence (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using ACTB polyclonal antibody (Cat # PAB9980) shows detection of a predominant band at ~42 KDa corresponding to ACTB (arrowhead) in various lysates. Lysates are as follows : HEK 293 (Lane 2), human lung carcinoma A-549 (Lane 3) and mouse brain (Lane 4) all using the 800 nm channel (green). Approximately 35 ug of each lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500. Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Molecular weight markers are shown in lane 1 using the 700 nm channel (red). IRDye™800 fluorescence image was captured using the Odyssey u Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | WB,ELISA,IF |
| Shipping condition: | Dry Ice |
| Publications: | Proteomic identification of brain proteins that interact with dynein light chain LC8.Navarro-Lerida I, Martinez Moreno M, Roncal F, Gavilanes F, Albar JP, Rodriguez-Crespo I. Proteomics. 2004 Feb;4(2):339-46. |