PPP1R13B polyclonal antibody View larger

PPP1R13B polyclonal antibody

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Data sheet of PPP1R13B polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse
Host speciesRabbit
ApplicationsELISA,WB-Tr

More info about PPP1R13B polyclonal antibody

Brand: Abnova
Reference: PAB9974
Product name: PPP1R13B polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of PPP1R13B.
Gene id: 23368
Gene name: PPP1R13B
Gene alias: ASPP1|KIAA0771|p53BP2-like|p85
Gene description: protein phosphatase 1, regulatory (inhibitor) subunit 13B
Immunogen: A synthetic peptide corresponding to internal region of human PPP1R13B.
Protein accession: NP_056131;Q96KQ4
Form: Liquid
Recommend dilutions: ELISA (1:2500-1:10000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human,Mouse
Application image: PAB9974-51-7-1.jpg
Application image note: Western blot using PPP1R13B polyclonal antibody (Cat # PAB9974) to detect over-expressed PPP1R13B in MCF-7 cells (Lane 2, arrowhead).
Lane 1 is a non-transfected control.
Lane 3 is MCF-7 cells over-expressing TP53BP2.
Cell extracts were electrophoresed and transferred to nitrocellulose.
The membrane was probed with the primary antibody at a 1 : 1,000 dilution.
The identity of the lower MW band at approximately 50 KDa is unknown.
Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.
Applications: ELISA,WB-Tr
Shipping condition: Dry Ice
Publications: The ASPP family: deciding between life and death after DNA damage.Slee EA, Lu X.
Toxicol Lett. 2003 Apr 4;139(2-3):81-7.

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