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Data sheet of AP3D1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Dog,Human,Mouse,Rat |
| Host species | Goat |
| Applications | IP,ELISA,WB-Ce |
More info about AP3D1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9965 |
| Product name: | AP3D1 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of AP3D1. |
| Gene id: | 8943 |
| Gene name: | AP3D1 |
| Gene alias: | ADTD|hBLVR |
| Gene description: | adaptor-related protein complex 3, delta 1 subunit |
| Immunogen: | A synthetic peptide corresponding to amino acids 686-698 of human AP3D1. |
| Protein accession: | O14617;NP_003929 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using AP3D1 polyclonal antibody (Cat # PAB9965) shows detection of a 130 KDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specificbinding. Approximately 20 ug of lysate was run ona SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP3D1 polyclonal antibody. Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-GoatIgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection using a 30-sec exposure time. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Characterization of the adaptor-related protein complex, AP-3.Simpson F, Peden AA, Christopoulou L, Robinson MS. J Cell Biol. 1997 May 19;137(4):835-45. |