Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Dog,Human,Monkey,Mouse,Rat |
Host species | Rabbit |
Applications | ELISA,IHC,WB-Tr |
Brand: | Abnova |
Reference: | PAB9962 |
Product name: | ABCB1 polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ABCB1. |
Gene id: | 5243 |
Gene name: | ABCB1 |
Gene alias: | ABC20|CD243|CLCS|GP170|MDR1|MGC163296|P-GP|PGY1 |
Gene description: | ATP-binding cassette, sub-family B (MDR/TAP), member 1 |
Immunogen: | A synthetic peptide corresponding to amino acids 262-277 of human ABCB1. |
Protein accession: | NP_000918;P08183 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Dog,Human,Monkey,Mouse,Rat |
Application image: | ![]() |
Application image note: | Western blot using ABCB1 polyclonal antibody (Cat # PAB9962) shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1 : 600, followed by Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG at 1 : 10,000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD. |
Applications: | ELISA,IHC,WB-Tr |
Shipping condition: | Dry Ice |
Publications: | The remarkable transport mechanism of P-glycoprotein: a multidrug transporter.Al-Shawi MK, Omote H. J Bioenerg Biomembr. 2005 Dec;37(6):489-96. |