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ABCB1 polyclonal antibody

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Data sheet of ABCB1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityDog,Human,Monkey,Mouse,Rat
Host speciesRabbit
ApplicationsELISA,IHC,WB-Tr

More info about ABCB1 polyclonal antibody

Brand: Abnova
Reference: PAB9962
Product name: ABCB1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of ABCB1.
Gene id: 5243
Gene name: ABCB1
Gene alias: ABC20|CD243|CLCS|GP170|MDR1|MGC163296|P-GP|PGY1
Gene description: ATP-binding cassette, sub-family B (MDR/TAP), member 1
Immunogen: A synthetic peptide corresponding to amino acids 262-277 of human ABCB1.
Protein accession: NP_000918;P08183
Form: Liquid
Recommend dilutions: ELISA (1:10000-1:50000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Dog,Human,Monkey,Mouse,Rat
Application image: PAB9962-51-158-1.jpg
Application image note: Western blot using ABCB1 polyclonal antibody (Cat # PAB9962) shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1.
The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose.
The membrane was probed with the primary antibody diluted to 1 : 600, followed by Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG at 1 : 10,000.
Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
Applications: ELISA,IHC,WB-Tr
Shipping condition: Dry Ice
Publications: The remarkable transport mechanism of P-glycoprotein: a multidrug transporter.Al-Shawi MK, Omote H.
J Bioenerg Biomembr. 2005 Dec;37(6):489-96.

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