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Data sheet of MYL6 (phospho S19/20) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce,IHC-P |
More info about MYL6 (phospho S19/20) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9961 |
| Product name: | MYL6 (phospho S19/20) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of MYL6. |
| Gene id: | 4637 |
| Gene name: | MYL6 |
| Gene alias: | ESMLC|LC17-GI|LC17-NM|LC17A|LC17B|MLC1SM|MLC3NM|MLC3SM |
| Gene description: | myosin, light chain 6, alkali, smooth muscle and non-muscle |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S19/S20 of human MYL6. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:120000-1:135000) Western Blot (1:1000-1:5000) Immunohistochemistry (2.5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is phosphospecific and detect monophosphorylated and diphosphorylated forms of the protein. This phosphospecific polyclonal antibody is specific to the phosphorylated pS19/pS20 form of the protein, depending on the source origin of the protein. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 1000 dilution to detect myosin light chain by Western blot on NIH/3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown.The phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain. Personal communication. J. Stull. UT Southwestern Medical Center. |
| Applications: | IP,ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |