| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce,IHC-P |
| Brand: | Abnova |
| Reference: | PAB9943 |
| Product name: | CCNB1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant CCNB1. |
| Gene id: | 891 |
| Gene name: | CCNB1 |
| Gene alias: | CCNB |
| Gene description: | cyclin B1 |
| Immunogen: | Recombinant GST fusion protein corresponding to full length human CCNB1. |
| Protein accession: | NP_114172;P14635 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse,Rat |
| Application image: | ![]() |
| Application image note: | Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) antibody shows detection of human CCNB1 present in asynchronous HN30 cell lysates. HN30 cells are from head and neck cancer tumors that over express CCNB1 and D1. Comparison to a molecular weight marker indicates a band of ~62 KDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG and chemiluminescence reagent with a 1-min exposure time. Personal communication, Luca Cote, Temple University, Philadelphia, PA. |
| Applications: | IP,ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.Giordano A, Whyte P, Harlow E, Franza BR Jr, Beach D, Draetta G. Cell. 1989 Sep 8;58(5):981-90. |