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Data sheet of TUBA1B polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Birds,Human |
| Host species | Rabbit |
| Applications | WB,ELISA,IF |
More info about TUBA1B polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9934 |
| Product name: | TUBA1B polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of TUBA1B. |
| Gene id: | 10376 |
| Gene name: | TUBA1B |
| Gene alias: | K-ALPHA-1 |
| Gene description: | tubulin, alpha 1b |
| Immunogen: | A synthetic peptide corresponding to amino acids 427-441 of human TUBA1B. |
| Protein accession: | P68363;NP006000 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000 Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Birds,Human |
| Application image: |  |
| Application image note: | Western blot using TUBA1B polyclonal antibody (Cat # PAB9934) shows detection of a predominant band at ~50 KDa corresponding to TUBA1B (arrowhead). Lanes correspond to whole cell lysates from mouse brain (Lane 1), rat brain (Lane 2), A-431 cells (Lane 3), Jurkat cells (Lane 4) and HeLa cells (Lane 5). Approximately 35 &mciro;g of each lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 1,200. Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rb IgG [H&L] MX10 for 45 min at room temperature. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | WB,ELISA,IF |
| Shipping condition: | Dry Ice |
| Publications: | Structural features and restricted expression of a human alpha-tubulin gene.Hall JL, Cowan NJ. Nucleic Acids Res. 1985 Jan 11;13(1):207-23. |