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Data sheet of AKT1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P,IF |
More info about AKT1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9931 |
| Product name: | AKT1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of AKT1. |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to amino acids 460-480 of AKT1. |
| Immunogen sequence/protein sequence: | CRPHFPQFSYSASGTA |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:500-1:2000) Immunofluorescence (1:100-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human,Mouse,Rat,Chicken |
| Reactivity: | Chicken,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunofluorescence microscopy of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT1 construct. AKT1 polyclonal antibody (Cat # PAB9931) staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin. |
| Applications: | ELISA,WB-Ce,IHC-P,IF |
| Shipping condition: | Dry Ice |
| Publications: | Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture.Alessi DR. Biochem Soc Trans. 2001 May;29(Pt 2):1-14. |