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Data sheet of JUB polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce,WB-Tr |
More info about JUB polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9930 |
| Product name: | JUB polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of JUB. |
| Gene id: | 84962 |
| Gene name: | JUB |
| Gene alias: | Ajuba|MGC15563 |
| Gene description: | jub, ajuba homolog (Xenopus laevis) |
| Immunogen: | A synthetic peptide corresponding to amino acids 224-239 of human JUB. |
| Protein accession: | Q96IF1;NP_116265 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:20000-1:80000) Western Blot (1:500-1:2500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of a 57 KDa band consistent with the expected MW for JUB (arrowhead). Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A-431, 4) Jurkat and 5) 293 whole cell lysates. Immunoprecipitation of JUB followed by western blotting may result in cleaner background staining. Approximately 5 ug ofeach preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of JUB polyclonal antibody. Detection occurred using a 1 : 5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. Achemiluminescence system was used for signal detection using a 60-sec exposure time. Personal Communication. E. Pugacheva, Fox Chase Cancer Center, Philadelphia, PA. |
| Applications: | IP,ELISA,WB-Ce,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner.Goyal RK, Lin P, Kanungo J, Payne AS, Muslin AJ, Longmore GD. Mol Cell Biol. 1999 Jun;19(6):4379-89. |