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AHSA1 polyclonal antibody

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Data sheet of AHSA1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityChimpanzee,Human
Host speciesRabbit
ApplicationsELISA,IHC,WB-Tr

More info about AHSA1 polyclonal antibody

Brand: Abnova
Reference: PAB9927
Product name: AHSA1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of AHSA1.
Gene id: 10598
Gene name: AHSA1
Gene alias: AHA1|C14orf3|p38
Gene description: AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Immunogen: A synthetic peptide corresponding to internal region of human AHSA1.
Form: Liquid
Recommend dilutions: ELISA (1:35000-1:185000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Chimpanzee,Human
Application image: PAB9927-51-83-1.jpg
Application image note: Western blot using AHSA1 polyclonal antibody (Cat # PAB9927) shows detection of AHSA1 in COS-7 cells.
For Lanes 2 and 4, COS-7 cells were transfected with pcDNA3-FLAG-AHSA1.
For Lanes 1 and 3, COS-7 cells were not transfected.
Extracts (40 ug per lane) were electrophoresed and transferred to nitrocellulose.
The membrane was probed with anti-AHSA1 (lanes 1 and 2, 1 : 2,000 dilution) or anti-FLAG (lanes 3 and 4).
The lower band seen in anti-AHSA1 blotting (arrowhead) is endogenous AHSA1.
Personal Communication, Brad Scroggins, CCR-NCI, Bethesda, MD.
Applications: ELISA,IHC,WB-Tr
Shipping condition: Dry Ice
Publications: Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1.Panaretou B, Siligardi G, Meyer P, Maloney A, Sullivan JK, Singh S, Millson SH, Clarke PA, Naaby-Hansen S, Stein R, Cramer R, Mollapour M, Workman P, Piper PW, Pearl LH, Prodromou C.
Mol Cell. 2002 Dec;10(6):1307-18.

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