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Data sheet of ABCB5 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Tr |
More info about ABCB5 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9925 |
| Product name: | ABCB5 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ABCB5. |
| Gene id: | 340273 |
| Gene name: | ABCB5 |
| Gene alias: | ABCB5alpha|ABCB5beta|EST422562 |
| Gene description: | ATP-binding cassette, sub-family B (MDR/TAP), member 5 |
| Immunogen: | A synthetic peptide corresponding to internal region of human ABCB5. |
| Protein accession: | AAW31630;Q515Q7 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:80000-1:85000) Western Blot (1:10000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using ABCB5 polyclonal antibody (Cat # PAB9925) shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1 : 10,000 dilution in 5% milk in TBST at 4°C, overnight. Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette.Chen KG, Gottesman MM. Biotechniques. 2005 Feb;38(2):198, 200, 202, 204. |