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Data sheet of ATR polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Clawed frog,Dog,Human,Monkey,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA |
More info about ATR polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9924 |
| Product name: | ATR polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ATR. |
| Gene id: | 545 |
| Gene name: | ATR |
| Gene alias: | FRP1|MEC1|SCKL|SCKL1 |
| Gene description: | ataxia telangiectasia and Rad3 related |
| Immunogen: | A synthetic peptide corresponding to amino acids 262-277 of human ATR. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:70000) Western Blot(1:1000-1:5000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Clawed frog,Dog,Human,Monkey,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using ATR polyclonal antibody (Cat # PAB9924) in HeLa cell nuclear extract (Lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ug peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kDa ATR. The primary antibody was used at a 1 : 1,400 dilution. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint.Olson E, Nievera CJ, Klimovich V, Fanning E, Wu X. J Biol Chem. 2006 Dec 22;281(51):39517-33. Epub 2006 Oct 10. |