| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat |
| Host species | Rabbit |
| Applications | WB-Ce |
| Brand: | Abnova |
| Reference: | PAB9647 |
| Product name: | Raf1 (phospho S301) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Raf1. |
| Gene id: | 24703 |
| Gene name: | Raf1 |
| Gene alias: | - |
| Gene description: | v-raf-leukemia viral oncogene 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S301 of rat Raf1. |
| Protein accession: | P11345 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Rat |
| Reactivity: | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat |
| Application image: | ![]() |
| Application image note: | Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Raf1 (phospho S301) polyclonal antibody (Cat # PAB9647). The immunolabeling of Raf1 is completely eliminated by treatment with lambda-Ptase. |
| Applications: | WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Untying the regulation of the Raf-1 kinase.Dhillon AS, Kolch W. Arch Biochem Biophys. 2002 Aug 1;404(1):3-9. |