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Data sheet of Polo-Like Kinase (phospho T210) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Clawed frog,Dog,Human,Mouse,Primates,Rat,Zebra fish |
| Host species | Rabbit |
| Applications | WB-Ti |
More info about Polo-Like Kinase (phospho T210) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9614 |
| Product name: | Polo-Like Kinase (phospho T210) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Polo-Like Kinase. |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T210 of human and rat Polo-Like Kinase. |
| Protein accession: | P53350 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human,Rat |
| Reactivity: | Bovine,Clawed frog,Dog,Human,Mouse,Primates,Rat,Zebra fish |
| Application image: |  |
| Application image note: | Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k Polo-Like Kinase protein phosphorylated at Thr210 (control). The phosphospecificity of this abeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Polo-Like Kinase (phospho T210) polyclonal antibody (Cat # PAB9614). The mmunolabeling is completely eliminated by treatment with lambda-Ptase. |
| Applications: | WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH. J Biol Chem. 2004 Aug 27;279(35):36841-54. Epub 2004 Jun 21. |