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Data sheet of MAP1LC3A (phospho S12) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,Dot-Pep,WB-Ce |
More info about MAP1LC3A (phospho S12) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB8066 |
| Product name: | MAP1LC3A (phospho S12) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP1LC3A. |
| Gene id: | 84557 |
| Gene name: | MAP1LC3A |
| Gene alias: | LC3|LC3A|MAP1ALC3|MAP1BLC3 |
| Gene description: | microtubule-associated protein 1 light chain 3 alpha |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S12 of human MAP1LC3A. |
| Protein accession: | NP_115903;Q5JWU0 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000) Dot Blot (1:100-500) Western Blot (1:100-500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunoblots of Phospho-MAP1LC3A in CHO cell culture. MAP1LC3 and MAP1LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066). MAP1LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of MAP1LC3 with alanine. WT = wildtype MAP1LC3-transfected cell lysates; S12A = MAP1LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no MAP1LC3 gene. Molecular size: MAP1LC3-I = 16 KDa, and MAP1LC3-II = 14 KDa. |
| Applications: | ELISA,Dot-Pep,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Autophagy in metazoans: cell survival in the land of plenty.Lum JJ, DeBerardinis RJ, Thompson CB. Nat Rev Mol Cell Biol. 2005 Jun;6(6):439-48. |