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| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
| Brand: | Abnova |
| Reference: | PAB7922 |
| Product name: | NFKBIA (phospho Y42) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA. |
| Gene id: | 4792 |
| Gene name: | NFKBIA |
| Gene alias: | IKBA|MAD-3|NFKBI |
| Gene description: | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y42 of human NFKBIA. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000) Immunoprecipitation (5-10 ul) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This peptide sequence has low homology to other IkB proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif. |
| Reactivity: | Human,Mouse,Rat |
| Application image: | ![]() |
| Application image note: | Western blot analysis of A-431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with NFKBIA (phospho Y42) polyclonal antibody (Cat # PAB7922). The latter antibodies were used in the presence of no blocking peptide (lane 1), phospho-NFKBIA (Tyr-42) peptide (lane 2), phospho-NFKBIA (Tyr-305) peptide (lane 3), or BSA conjugated to phospho-tyrosine (lane 4). Peptides and BSA-pTyr were used at 1 mg/ml. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Regulation of COX-2 mediated signaling by alpha3 type IV noncollagenous domain in tumor angiogenesis.Boosani CS, Mannam AP, Cosgrove D, Silva R, Hodivala-Dilke KM, Keshamouni VG, Sudhakar A. Blood. 2007 Aug 15;110(4):1168-77. Epub 2007 Apr 10. |