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Data sheet of CFL1 (phospho S3) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IF |
More info about CFL1 (phospho S3) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB7900 |
| Product name: | CFL1 (phospho S3) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of CFL1. |
| Gene id: | 1072 |
| Gene name: | CFL1 |
| Gene alias: | CFL |
| Gene description: | cofilin 1 (non-muscle) |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to resdiues surrounding S3 of human CFL1. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000) Immunocytochemistry (1:50) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot of Jurkat cells expressing CFL1. Blots were treated with (lanes 2, 3) or without (lane 1) lambda phosphatase for 1 hr at 37 °C. In lane 3, phosphatase treatments were performed in the presence of phospho-CFL1 (Ser-3) peptide. Blots were probed with CFL1 (phospho S3) polyclonal antibody (Cat # PAB7900) (lane 1). Phosphatase treatment removes Ser3 phosphorylation of CFL1, but this phosphatase activity is reduced in the presence of peptide. |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Proteins of the ADF/cofilin family: essential regulators of actin dynamics.Bamburg JR. Annu Rev Cell Dev Biol. 1999;15:185-230. |