| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,WB-Ti,IHC-P,IF |
| Brand: | Abnova |
| Reference: | PAB7499 |
| Product name: | VPS35 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of VPS35. |
| Gene id: | 55737 |
| Gene name: | VPS35 |
| Gene alias: | DKFZp434E1211|DKFZp434P1672|FLJ10752|FLJ13588|FLJ20388|MEM3 |
| Gene description: | vacuolar protein sorting 35 homolog (S. cerevisiae) |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human VPS35. |
| Immunogen sequence/protein sequence: | C-SPESEGPIYEGLIL |
| Protein accession: | NP_060676.2 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:32000) Western Blot (0.01-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-10 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Specificity: | There is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical. |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | VPS35 polyclonal antibody (Cat # PAB7499) staining of paraffin embedded human testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A) Tris/EDTA buffer pH9 at 3 ug/mL or B) Citrate buffer pH6 at 10ug/mL ., HRP-staining. |
| Applications: | ELISA,WB-Ti,IHC-P,IF |
| Shipping condition: | Dry Ice |
| Publications: | EHD1 interacts with retromer to stabilize SNX1 tubules and facilitate endosome-to-Golgi retrieval.Gokool S, Tattersall D, Seaman MN. Traffic. 2007 Dec;8(12):1873-86. Epub 2007 Oct 7. |