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Data sheet of NANOG polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,S-ELISA,WB-Ce,IHC-P |
More info about NANOG polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB7232 |
| Product name: | NANOG polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of NANOG. |
| Gene id: | 79923 |
| Gene name: | NANOG |
| Gene alias: | - |
| Gene description: | Nanog homeobox |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human NANOG. |
| Immunogen sequence/protein sequence: | C-DLFLNYSMNMQPED |
| Protein accession: | NP_079141.1 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:128000) Sandwich ELISA (5-10 ug/mL) Western blot (0.05-0.1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | NANOG polyclonal antibody (Cat # PAB7232) (0.05 ug/mL) staining of HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| Applications: | ELISA,S-ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer.Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H. Genes Cells. 2006 Sep;11(9):1115-23. |