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Data sheet of ITCH polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,WB-Ti |
More info about ITCH polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB7215 |
| Product name: | ITCH polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of ITCH. |
| Gene id: | 83737 |
| Gene name: | ITCH |
| Gene alias: | AIF4|AIP4|NAPP1|dJ468O1.1 |
| Gene description: | itchy E3 ubiquitin protein ligase homolog (mouse) |
| Immunogen: | A synthetic peptide corresponding to human ITCH. |
| Immunogen sequence/protein sequence: | C-EIKSHDLKPNGGN |
| Protein accession: | NP_113671.3 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:32000) Western Blot (0.03-0.1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | ITCH polyclonal antibody (Cat # PAB7215) (0.03 ug/mL) staining of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination.Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A. Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19. |