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GAPDH polyclonal antibody

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Data sheet of GAPDH polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesGoat
ApplicationsELISA,IHC-Fr,WB-Ti

More info about GAPDH polyclonal antibody

Brand: Abnova
Reference: PAB6936
Product name: GAPDH polyclonal antibody
Product description: Goat polyclonal antibody raised against synthetic peptide of GAPDH.
Gene id: 2597
Gene name: GAPDH
Gene alias: G3PD|GAPD|MGC88685
Gene description: glyceraldehyde-3-phosphate dehydrogenase
Immunogen: A synthetic peptide corresponding to internal region of human GAPDH.
Immunogen sequence/protein sequence: C-GVNHEKYDNSLK
Protein accession: NP_0020.37.2
Form: Liquid
Concentration: 0.5 mg/mL
Recommend dilutions: Immunohistochemistry (Frozen sections) (10-20 ug/mL)
ELISA (1:4000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage instruction: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Goat
Antigen species / target species: Human
Specificity: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Reactivity: Human,Mouse,Rat
Application image: PAB6936-47-5-1.jpg
Application image note: GAPDH polyclonal antibody (Cat # PAB6936) (0.1 ug/mL) staining of human tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Applications: ELISA,IHC-Fr,WB-Ti
Shipping condition: Dry Ice
Publications: Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase.Ismail SA, Park HW.
Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1508-13. Epub 2005 Oct 19.

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